Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTTEYVAEILNELHNSAAYISN---EEADQLADHILSSHQIFTAGAGRSGLMAKSFAMRLMHMGFNAHIVGEIL--------------------TPPLAEGDLVIIGSGSGETKSLIHTAAKAKSLHGIVAALTINPESSIGKQ---ADLIIRMPGSPKDQSNGSYKTIQPMGSLFEQTLLLFYDAVILKLMEKKGLDSETMFTHHANLE 3TRJ Chain:A ((8-191)) ---NSYFESSIQAKIETANALPPAIAQAAKAMVSCLENGGKVLVCGNGSSGVIAQHFTSKLLN-PLPAIALTGDVATITAVGNHYGFSQIFAKQVAALGNEDDILLVITTSGDSENILSAVEEAHDLEMKVIALTGGSGGALQNMYNTDDIELRVPSDNIAN----IQEN-HF-----LIVHCLCDIIDQK--------------------

General information: TITO was launched using: RESULT: Template: 3TRJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 737 -88664 -120.30 -587.18 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -120.30 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_3TRJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TRJ-query.scw PDB file : Tito_Scwrl_3TRJ.pdb: