Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMIIIPNNEIAKHMLTDFFAKHWGTPEM-A--ISSG-IF-RC---DELDGFAAVN-------ESGEIIGCITYTIDG-----KDCEI--ISLDSMIENKGIGTALLQQVEEKAKHAHCQRIKLITTNDNVNAIAFYQKRGYQFAAVFPNAVEKARRLKPEIPEVAENGILIRDEILFSKVID 2B5G Chain:A ((3-151)) KFVIRPATAADCSDILRLIKELAKYE--QVILTEKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSE-----------------------------------

General information: TITO was launched using: RESULT: Template: 2B5G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 444 -82637 -186.12 -671.84 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -186.12 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_2B5G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B5G-query.scw PDB file : Tito_Scwrl_2B5G.pdb: