Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTIKKDFIPVSNDNRPGYAMAPA-----YITVHNTAN-------------TAKGADAKMHAKFVKNPNTSESWHFTVDDS-VIYQHLPIDENGWHAGDGT---NGTGNRKSIGIEICENADGDF-EKATSNAQWLIRKLMKENNIPLNRVVPHKKWSGKECPRKLLDHWNSFLNGISSSDTPPKETSPSYPLPSGVIKLTSPYRKGTNILQLQKALAVLHFYPDKGAKNNGIDGVYGPKTANAVKRFQLMNGLTADGIYGPKTKAKLKSKLK 1J3G Chain:A ((1-187)) MLLDEGWLAEARRVPSPHYDCRPDDENPSLLVVHNISLPPGEFGGPWIDALFTGTIDPNAHPYFAGIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSSYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTNALITRYPAIANNMTGH------------------------CNIAPERKTDPGPSFD--------------------------------------------------------WARFRALVTPSSHKEMT------

General information: TITO was launched using: RESULT: Template: 1J3G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 741 21164 28.56 129.05 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 28.56 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1J3G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J3G-query.scw PDB file : Tito_Scwrl_1J3G.pdb: