Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNKMLVAIDGSDMSAKALDAAVHLAKEQQAELSILHVGREAVVTTSSLTGIVYVPEHFIDEIRNEVKKEGLKILENAKEKAAEKGVQAETI-----YANGEPAHEILNHAKEKGVSLIVVGSRGISGLKEMMLGSVSHKVSQLSTCPVLIVR 4WNY Chain:A ((1-146)) MYSIILVALDGSQTASHALDAALELAADAHARLVPVYVVDM----FDT---PGY--DP--SILVDAFREEGRRVLDDAQARMTRRGVAGAPRLVEVE---EDVAERLERAAREIGASLIVMGTH-----------SVAERLLRHARCPVLMIP

General information: TITO was launched using: RESULT: Template: 4WNY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 525 -36407 -69.35 -295.99 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -69.35 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.717

(partial model without unconserved sides chains): PDB file : Tito_4WNY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WNY-query.scw PDB file : Tito_Scwrl_4WNY.pdb: