Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADYLVIVESPAKAKTIEKYLGKK-------------------FKVKASMGHVRDLPKSQ-M---GVDTEN-DYEPRY-ITIRGKGPVLKELKQAAKKAKKVYLAADPDREGEAIAWHLANSLELDQSDKLRVVFNEITKEAVKESFKTPRKIDMDLVNAQQARRILDRLVGYNISPILWKKVK-KGLSAGRVQSIALRIIIDREKEINNFKPEEYWTIDGNFLKGKKKFQANFYGVNGKKKKLSTADDVKEVMSAIKGKSFDVTDVTKKERLRNPAAPFTTSSLQQEAARKLNYRTRKTMMLAQQLYEGITLGKQGTVGLITYMRTDSTRIADSAILEASNYIKETYGPEFSRNHKRSDKNAKGAQDAHEAIRPTSAMRSPQEV-----------KEYLTRDQLRLYRLIWERFIASQMTPAVLDTMRVDLDNNGVNFRANGSKIK----FHGFMKVYVESNDDNTEEKENILPDLKKGDKVQSESLEQRQHFTQPPPRYTEARLVKTLEEIGIGRPSTYSPTLDTIQRRNYVSLTNKRFIPTELGEIVNEMIEEYFPEILDVKFTANMESELDEVEHGEVEWVKVIDEFYKRFEPNVIKADAEMEKIEIKDEPAGIDCDLCGAPMVYKMGKYGKFLACSRFPDCRNTKAIVKEIGVTCPKCEKGHVIERKSKKKRIFYGCDRYPDCDYVSWDKPVERACPKCEKRALVEKKLKKGVQVQCTNCDYKESTQQ 1GKU Chain:B ((505-1053)) -KPALFIVESPTKARQISRFFGKPSVKVLDGAVVYEIPMQKYVLMVTASIGHVVDLITNRGFHGVLVNGRFVPVYASIK---DNSRSRIEALRKLAHDAEFVIVGTDPDTEGEKIAWDLKNLLSGC-GAVKRAEFHEVTRRAILEALESLRDVDENLVKAQVVRRIEDRWIGFVLSQKLWERFNNRNLSAGRAQTLVLGWIIDRFQESRERRKIAIVR----------DFDLVLE---------HDEE----------EFDLTIKLVEEREELRTPLPPYTTETMLSDANRILKFSVKQTMQIAQELFEN---------GLITYHRTDSTRVSDVGQRIAKEYLGD----DFV---GRE-W---GESGAHECIRPTRPLT-RDDVQRLIQEGVLVVEG-LRWEHFALYDLIFRRFMASQCRPFKVVVKKYSIEFDGKTAEEERIVRAEGRAYELYRAVWVK----------NELP---TGT-FRV---KAEVKSVPKVLPFTQSEIIQMMKERGIGRPSTYATIVDRLFMRNYVVEKYGRMIPTKLGIDVFRFLVRRYAKFVSEDRTRDLESRMDAIERGELDYLKALEDMYAEIKSI---------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -64705 for 3908 contacts (-16.6/contact) + 2D Compatibility (PS) -51127 + (NN) -8081 + (LL) 11760 1D Compatibility (HY) -35600 + (ID) 8050 Total energy: -155803.0 ( -39.87 by residue) QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_1GKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GKU-query.scw PDB file : Tito_Scwrl_1GKU.pdb: