Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVLWVAAVIALACLNVIQFIMKKKRDGNLAYTADQLSYMLSRDSAGQILLPTDDHALKDLLVNINLIVENRQQISAQFAKTEQSMKRMLTNMSHDLKTPLTVILGYIEAIQSDPNMPDEERERLLGKLRQKTNELIQMINSFFDLAKLESEDKEIPITKVHMNDICKRNILHYYDAVQSKGFQAAIDIPDTPVYAQANEEALDRILQNLLSNAIQYGAAGKLIGLTLSYDERNIAITVWDRGKGISETDQQRVFERLYTLEESRNKAFQGSGLGLTITKRLTEKMGGIISVQSKPYERTAFTITLKRMTY 4Q20 Chain:A ((534-758)) ------------------------------------------------------------------------------LAEAERLKRDFVGNVSYELRTPLTTIIGYSELLERAD---SERGRNHVAAVRAAATQLARSIDDVLDMAQIDAGEMALEIEDIRVSDLLLNAQERALKDAQLGGVTLAVECEEDVGLIRGDGKRLAQTLDHLVENALRQTPPGGRVTLSARRALGEVRLDVSDTGRGVPFHVQAHIFDRFVGRD------RGGPGLGLALVKALVELHGGWVALESEPGNGSTFTCHLPETQ-

General information: TITO was launched using: RESULT: Template: 4Q20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 970 -81654 -84.18 -366.16 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -84.18 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_4Q20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q20-query.scw PDB file : Tito_Scwrl_4Q20.pdb: