Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIAL-TDLMKELNITAATVQWLTTGYLLVLGILVPVSGLLLQWF-TTRQLFTVSLIFSILGTFIAALAP-SFSFLLAARIVQALGTGLLLPLMFNTILVIFPPH-KRGAAMGTIGLVIMFAPAIGPTFSGLVLEH--------LNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLSIILSTI-------GFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLVLT---------AFASGLVLLPGGILNGFMSPVTGRLFDKYGP-KWLVIPGFVIVTVVLWFFSNVTT-----TSTAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH 4TPH Chain:A ((19-450)) -----------------------LIELWERFGYYGMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILVGLGNY-ALMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIILE-Y--EDVARV---FVYAAGVAVLGIFFH-L-------ERAGLIA---ALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSGLGLAMIAR---------MMGAYFVASGISQYLGGVVANFASVP------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1815 -295891 -163.03 -808.44 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -163.03 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: