Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MKGAAFVKKEGLKQKALEIGRVPTHLKLEIEDYGGDDKRAHFCWADPQDENTGIIVELGPDG------EL-ESLSRDIEPESGERLSEEKLEDIMRQFVETHHPGALSAFVREENDRAYGDKVRFSYVQMEAGLPLPMSGFMADVSLSGEIVYFRYYGEAGSIIKPKRV-A-DVEEALAFIKKDVEFDLLFEVLHRSVYKNGDDQPHLVYEPEGRAITVPADLVQEEQAVDDDDDYREPESFP-LPLFEGIREKADPDSMIGIENGFVKEREADLGDGRIGIVWR-NPDDPVYQPADKSMDSWFKGRTHQVL--KTIYNKETGKLEGVMSFMEKKGPLTVTLAECEKIALRF-LFALFPNADQYFRIRYDEKDEEENAVAGFTFEAHCHGVPLRFGQIRICVSRQTGYITVYMGPDIDPNKLATIDPVPAISVEQAKSIFWQHFKVELGWEREYGDDEEHSYRLVYKP-VYPHFIDAHTGEPVFSIW 5COZ Chain:A ((12-358)) DLNDYENVLNS-LDEE--QIGKLPQNIKCVVNDKLNIDSEINIWDATSYYVKSGKVKAINFSENKDKCYDLMEKLAKAINLNKDVCVQSHRSENGNEIYLWDNNYTQDSIAIR--NDSALAE-------THDGKLAVSASKFG---------TYYSPFNDKDKFRTDKQLMFMSAEEAEELAVKTAKE-LEINVCEKNELYVLDDKNTLIFPE------------------DDTDKQNDTYVFFMFPDVYGIPYSRCPEN----------EALTGYANQENHLVIAMDEKGISFLDIPPLYDWVETTETGEILHPSSILSKEVDKLKKYVTS---------GDIEVSEISLEYMLFADK-------------------------------------------------------------NETYDIKPVWVVYYYQNQLV-----------------TGENSY---TQKMALYDVYDAYTGEE-YRIQ

General information: TITO was launched using: RESULT: Template: 5COZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1714 -53469 -31.20 -161.54 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.20 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.157

(partial model without unconserved sides chains): PDB file : Tito_5COZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5COZ-query.scw PDB file : Tito_Scwrl_5COZ.pdb: