Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNKQLKKKAQEIGNVPTHYELEIEDYDQKQKKYGQA------YFIWKDPKDPEKHITVELRNDGALLTF-STTVHSETDKKLPDAELKLTALQFAAANHPGTFMNFHFQGKEERGQHIRFVYTKMELGLPIPNSGFLIDMTRSGQIVHFLYYGEGHKAEVPTEFVAKEKVVSHYLNTMSFELMYDVIDGEQEPRLVYEPILPGYSYPADVDEIVPDQHIADERIENTAPLPPLQNKEEV--DIFALLGFTSDMQKVSERDFGEQI-GSTWRRGAAPERKDLSIGSYFETRNKNTIKMKTDKRTGKLKAAL--------SFMDWRNNLQCSTEECQEIALQFL----------YALYPRAAEFFRVNPVRIDERGRVRNHFSVWYKGVPLRFGAARIIVNPETGLIDAFMAPDIE-PEQLEAINHRPDVSAE--EAKEAFLAAFD----VKLEWQPDFTAGSDQHCKLVYKPVYPSYIDAHIRKKKRL 4B28 Chain:A ((32-468)) RKIDPSRGATLGDGSPNDMNRVEIGPTQLAFAEWHTARLDLPDLAAMRRFRHRRLTDHVVARGYAGLLMFDPLNIRYATDSTNMQLWNTHNPFRATLLCADGYMVMWDYKNSPFLSEFNP-----------------LVREQRAG--ADLFYFDRGDKVDVAADVFANE--VRILLRDHAPGLRRLAVD--KVMLHGLRALQAQGFEIMDGEEVTEKARSVKGPDEIRAMRCASHACEVAVRKMEDFARSKVGDGVTCENDIWAILHSENVRRG----------GEWIETR-----LLASGPRSNPWFQECGPRVCQRNEIISFDTDLVGAYGICTDISRSWWIGDQKPRADMIYAMQHGVEHIRTNMEMLKPGVMIPELSANTHV-LDAKFQKQKYGC-LMHGVGLCDEWPLVAYPDHAVAGAYDYPLEPGMTLCVEALISEEGGDFSIKLEDQVLIT--EDGYENLTKYPFDPALMG---------

General information: TITO was launched using: RESULT: Template: 4B28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2208 -91233 -41.32 -226.95 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -41.32 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.057

(partial model without unconserved sides chains): PDB file : Tito_4B28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B28-query.scw PDB file : Tito_Scwrl_4B28.pdb: