Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTENQNPLVLDKNEEISQQKADDIRLQLRQEPEVKRLAQQIDVKNQMELLEYGKEPAVEISKFSDRILGMMKTTSVTDSGTMLTQLGKIMDRFDKNDFDEPKGLMAKIFKRGGSMIEKIFKKYQTLGGEIEKIHVEISKYKDEMTKTNYTLDEMYENNIKYYMELEKYVVAGQMKLEEMQSILPSYEEKAASGNQLAQMQLDTLRNGIQALE-ERVYDLDMARMVA-------LQTAPQIRLLQRGNAKLI-GKINSAFIITIPIFKNGIIQAVTVKRQKLVADSMSELDRRTNEMLKRNAENISSQSVEIARMAGRPSIDIETIESSWNTIVSGMQETKQIEEENKRLREDG-----------ARRIAQLQDNIKKAALQQ------------------- 4O6M Chain:A ((29-370)) ------MRLAYVKNHEIYGEKLLGLTLRERIEKTLQRAGFDVRFFDELSLEE------------AEDYLIILEPVLILERDLLLEG--------------------RKILVSDG------FTVGYFFGGDFRTVF-----------------DGNLQSSIEKYLSL------NNLESYEIWAIKLSND----NLKTAEKLLLSSLIDGWIAREINRKVSLRISRLLADTSVTPNQITVFSFFLSLVGSALFLLNSYLTTLLAGVIIQLHSIIDGCDGEIARL-KFMESKYGAWLDGVLDRYSDFIIVFSITYVLSASNPVYWIIGFLAAFASLMIAYTGDKFVAAYMRTYSPEGFAIPITRDFRLLIIFACSVVNLPSLALVIIALLGNFEALRRIVALRSY

General information: TITO was launched using: RESULT: Template: 4O6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1261 -82980 -65.80 -285.15 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -65.80 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_4O6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O6M-query.scw PDB file : Tito_Scwrl_4O6M.pdb: