Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKRTVKNIAEMVKGTLANPQYEQTVIHGVATDTRKLEQHQLFIPLKGERFDGHSFVEQAFEAGVAAVLWDRSVPNPPENHAVILVDDTLTALQQLAKAYLQE-LGTRVIGVTGSNGKTTTKDMIHAVLGTQYRVHKTGGNFNNHIGLPLTVLAMPENTEIAVLEMGMSAKGEIDLLSRLANPDAAVITNIGESHMQDLGSREGIAEAKLEIINGLKEDGVLIYIGDEPLLQNAY----SCQTKTYGTGTHNDYQLQDVSQSEEGTHFTIK--GIENTFFIPILGKHNVMNAMAAIAAGAYFGIAPEDAAKGLSGLKVTGMRLELIKTDSGLSIINDAYNASPTSMKAAIQLTESLEGYGKKMLVLGDMLELGDLEETFHKECGAVISPDKIDRVFTYGKLGAFIAEGALKHFEKDRVSHYTEKKDLLQAVKEN----ASKGDLILFKASRGMKLEEIVKDLIESPLS 4QDI Chain:A ((18-471)) -EPWTAQQLQQATQGYWHKDQIPQTEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVVANGCQVAIVERPID---AEIAQLVVADTRLALGQLGAYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAEIREAAKSHQIMSFGEG--GDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQAQGAKGRLNFIQK-APHLFIDDTYNANPTSMRAAAQVLLQQ--NGIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAVGQFASAALEGAGLHS--TKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEK---

General information: TITO was launched using: RESULT: Template: 4QDI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2585 -275356 -106.52 -621.57 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -106.52 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_4QDI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QDI-query.scw PDB file : Tito_Scwrl_4QDI.pdb: