Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEALTFESKYALLYLGGGLLAMIYGLITFFMAFPAFTSRGNVIFTIKTGPSGEIFLRKRSVLFSEVKRLYMGRHQYSLKGIFFEDIIIEKTDGKIVRIPTWN--IITNPLFFEAVERYILPHLNEEAQNNWISQFTEVQRKAYLKEFENHPKL 2L6O Chain:A ((1-114)) MGAGQTPHPQ---------------------LIWPALLKQ--------QGCNELLPLRTNDDWQRFCADSKHLLQY--------GDKLVD-SNFHCFVLEEDAHWHPAAPLPPEGLNDLIRAHCATLGHCC-TSKMHLHSVMDAIDFLNALEG-

General information: TITO was launched using: RESULT: Template: 2L6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 437 -38312 -87.67 -342.07 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -87.67 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.180

(partial model without unconserved sides chains): PDB file : Tito_2L6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L6O-query.scw PDB file : Tito_Scwrl_2L6O.pdb: