Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKRETFNETFLKAARGEKADHTPVWYMRQAGRSQPEYRKLKE-KYGLFEITHQPELCAYVTRLPVEQYGVDAAILYKDIMTPLPSIGVDVEIKNGIGPVIDQPIRSLADIEKLGQIDPEQDVPYVLETIKLLVNEQLNVPLIGFSGAPFTLASYMI------EGGPSKNYNKTKAFMYSMPDAWNLLMSKLADMIIVYVKAQIEAGAKAIQIFDSWVGALNQADYRTYIKPVMNRIFSELAK-ENV-PLIMFGVGASHLAGDWHDLPLDVVGLDWRLGIDEARSK-GITKTVQGNLDPSILLAP-WEVIEQKTKEILDQGMESDGFIFNLGHGVFPDVSPEVLKKLTAFVHEYSQNKKMGQYS 2EJA Chain:A ((2-337)) -----PKNDLLLRSLRGEPIGRFPVWLMRQAGRYMPEYRKIRNRVKNFLELCKNVDLATEISLLPLKILGVDAIIIFSDILVPLEPLGVKVEFVEGEGPK----LSWSGKVSDLKKYDPS-QNAYVYEIIKRVKEAQDEVPVIGFAGAPFTLLSYLIEGGASKDFKST------KLFMWENPKEYKRLMDILTETVLAYLKEQIKAGADVVQIFDSWVNNLSLEDYGEYVYPYVNYLISELKDFSDTPVIYFFRGS-SSFIDLAVDYRADALSVDWSVDIPELFKIYD--KGFQGNLEPAV-LYASEEVIEEKTLGLLRRIPVKTRYVFNLGHGLAPDMELEKVKYLVDLVKSFPL--------

General information: TITO was launched using: RESULT: Template: 2EJA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1796 -261351 -145.52 -804.16 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -145.52 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_2EJA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EJA-query.scw PDB file : Tito_Scwrl_2EJA.pdb: