Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPYEVLEDPGIDAVIIVTPTSTHGDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKET-GVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGA-PPAE--F-I-KHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGHD-IRAEVIGTEGSIFIGTLRHQHVTILSAKGSSFDII-P-DFQTRFHEAYCLELQHFAECVRNGKTPI-VTDIDATINLEVGIAATNSFRNGMPVQLDVKRAYTGM 3CEA Chain:A ((5-345)) TRKPLRAAIIGLGRLGERHARHLVNKIQGVKLVAACALDSNQLEWAKNELGVETTYTNYKDMIDTENIDAIFIVAPTPFHPEMTIYAMNAGLNVFCEKPLGLDFNEVDEMAKVIKSHPNQIFQSGFMRRYDDSYRYAKKIVDNGDIGKIIYMRGYGIDPISGMESFTKFATEADSGGIFVDMNIHDIDLIRWFTGQDPVQAYGLTSNIAAPQLADIGEFETGVAQLKMSDGVIATLIGGRHAAHG-NQVELEVMGSNGWVRIGEHP-DLNRVTVFNDQGVVRPSLQSFGERFDTAFTDEVQDFVNNVIVGK-QPEVTVDDGIKALKIAKACQQSANIGKLVDIQ--------

General information: TITO was launched using: RESULT: Template: 3CEA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1825 -195143 -106.93 -589.56 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -106.93 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_3CEA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CEA-query.scw PDB file : Tito_Scwrl_3CEA.pdb: