Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGNEPHGGVLINRCDPACHF----------EGCACQAELDQLALSDLELIAIGGYSPLTGFLGEKDYHSVVKEMRLANGLPWSLPITLPVGEKTARQLSAGDHVKLVK-DGVTYGMITVTDIYQPDKTQEALSVFKTNDPAHPGVKKLLA-RPDYYIGGPITVSSLPD-KSFEQFYATPAETRAAFQKLGWKTIVGFQTRNPVHRAHEYIQKTALET-VDGLLLHPLVGETKSDDIPSDIRMESYQALLNHYYPKDRVMLSVFPAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVG------SYYGTYDAQNIFQSFTEEELGIKPLFFEHSFYCRKCGNMGTSKTCPHSPRDHIHLSGTKVRELLRQGKKPPKEFSRPEVAAVLIKGLHQQPVAIKQNSGELQ 1R6X Chain:A ((1-386)) ---PAPHGGILQDLIARDALKKNELLSEAQSSDI-LVWNLTPRQLCDIELILNGGFSPLTGFLNENDYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRIALFQDDEIPIAILTVQDVYKPNKTIEAEKVFRGD-PEHPAISYLFNVAGDYYVGGSLEAIQLPQHYDYPGLRKTPAQLRLEFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAKVLIHPVVGLTKPGDIDHHTRVRVYQEIIKRY-PNGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGKNSKGVDFYGPYDAQELVESYK-HELDIEVVPFRMVTYLPDEDRYAPIDQIDTTKTRTLNISGTELRRRLRVGGEIPEWFSYPEVVKILRES----------------

General information: TITO was launched using: RESULT: Template: 1R6X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2005 -184416 -91.98 -503.87 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -91.98 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_1R6X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R6X-query.scw PDB file : Tito_Scwrl_1R6X.pdb: