Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLYMSVDMEGISGLPDDTFVDSGKRNYERGRLIMTEEANYCIAEAFNSGCTEVLVNDSHSKMNNLMVEKLHP-EADLISGDVKPFSMVEGLDDTFRGALFLGYHARAST-PGVMSHSMIFGVRHFYINDRPVGELGLNAYVAGYYDVPVLMVAGDDRAAKEAEELIPNVTTAAVKQTISRSAVKCLSPAKAGRLLTEKTAFALQNKDKVKPLTPPDRPVLSIEFANYGQAEWANLMPGTEIKTGTTTVQFQAKDMLEAYQAMLVMTELAMRTSFC 1OZH Chain:A ((13-157)) -------------------------------------GADLVVSQLEAQGVRQVFGIPGAK--IDKVFDSLLDSSIR----------IIPVRHEANAAFMAAAVGRITGKAGVALVTSGPG-CSN-LIT---------GMATANSEGDPVVALGGAVKRADK------SMDTVAMFSPVTKYAIEVTAPDALAEVVSNAFRAAE------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1OZH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 681 -74932 -110.03 -550.97 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -110.03 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_1OZH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OZH-query.scw PDB file : Tito_Scwrl_1OZH.pdb: