Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYRAVFPMLIIVFALSGCTLSTINPMKKSRIDNIHHTQILFFSDENQIDQEAPYYDALLDLEKDYPEQIDKMKVYDKKEG---WEDEIETVPTLMVVDQRHVVVKIEGCVKKKEDIIKPLQHVLSK 1T00 Chain:A ((21-109)) -----------------------------------DKPVLVDFWAAWCGP-CRQIAPSLEAIAAEYGDKIEIVKLNIDENPGTAAKYGVMSIPTLNVYQGGEVAKTIVG-AKPKAAIVRDLEDFIA-

General information: TITO was launched using: RESULT: Template: 1T00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 356 -48055 -134.98 -558.77 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -134.98 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_1T00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T00-query.scw PDB file : Tito_Scwrl_1T00.pdb: