Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADTLERVTKIIVDRLGVDEADVKLEASFKEDLGADSLDVVELVMELEDEFDMEISDEDAEKIATVGDAVNYIQNQQ 4IHH Chain:I ((3-77)) MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPDEEAEKITTVQAAIDYING--

General information: TITO was launched using: RESULT: Template: 4IHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 257 -41512 -161.53 -553.49 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain I : 0.88 3D Compatibility (PKB) : -161.53 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.754

(partial model without unconserved sides chains): PDB file : Tito_4IHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IHH-query.scw PDB file : Tito_Scwrl_4IHH.pdb: