Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVIDDFEKLDIRTGTIVKAEEFPEARVPAIKLVIDFGTEIGIKQSSAQITKRYKPEGLINKQVIAVVNFPPRRIAGFKSEVLVLGGIPGQGDVVLLQPDQPVPNGTKIG 1MKH Chain:A ((2-107)) YVKFDDFAKLDLRVGKIIEVKDHPNADK-LYVVKVDLGDE--VRTLVAGLKKYYKPEELLNRYVVVVANLEPKKL-GIGSQGMLLAADDGE-RVALLMPDKEVKLGAKVR

General information: TITO was launched using: RESULT: Template: 1MKH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 453 -53681 -118.50 -511.24 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -118.50 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_1MKH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MKH-query.scw PDB file : Tito_Scwrl_1MKH.pdb: