Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MFKKGQKVIVDFTDEIGAVAKVDYRY--NQIEVKYPDGTYQVVGFHKVRKVED------------- 2EQU Chain:A ((78-151)) GSSGSSGFDFKAGEEVLARWTDCRYYPAKIEAINKEGTFTVQFYDGVIRCLKRMHIKAMPEDAKGQDWIALVKA

General information: TITO was launched using: RESULT: Template: 2EQU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 126 -942 -7.47 -18.46 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -7.47 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.601

(partial model without unconserved sides chains): PDB file : Tito_2EQU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EQU-query.scw PDB file : Tito_Scwrl_2EQU.pdb: