Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKYETNGYDTSIVYDYKEYPDVKYGRCDNCDYTLFKSSVKSGIFLRECRRCGMKKSI 1M9O Chain:A ((4-43)) ---MTTSSRYKTELCRTYSESGRCRYG--AKCQFAHGLGELRQAN--------------

General information: TITO was launched using: RESULT: Template: 1M9O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 116 -7116 -61.34 -177.89 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -61.34 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.211

(partial model without unconserved sides chains): PDB file : Tito_1M9O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M9O-query.scw PDB file : Tito_Scwrl_1M9O.pdb: