Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTI---GDVDIATDAAPDQVERLFQRTV-D---VGKEHGT---IIVLWE----DETYEVTTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAMTAD---------GKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLRAVRFMSQLGFTLSPETEEAIAKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEFP-----------FF----------------------------SLTSREELWAALL-INLGIVL------------KDAPLFLKAWKLPGKVIKEAIHIADT-FGQSL-----------DAMTMYRAGKKALLSAAK---ISQLRQNEKLDE-KKLKDIQYAYQNLP-------IKSLKDLDITGKDLLALRNRPAGKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH 3WFO Chain:A ((24-448)) -------FDDVAKVL-PREHYCFIVGGWVRDRILGEPVGYNIDVDFLTTADPVELAKNFAKRIGGHFFVFE----PTIASVVLHLPPYRYRFDFSPLKGK------------DLEKALIEDLKERDFTANAIAVNLDDVLSIGAKQTIVYDPTGGIKDLEQGLLRPVS--IENLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVLKSAVERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKD-----PLDEHTLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELL-KWGALFHDIGKPQTFVTFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHL-RPFFLREAFKKGELKRRGMANFWRECGDIAPHLFLLSIADAMASGDEEEDIKALMETIAELESFNRNEMK----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1405 28935 20.59 94.56 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 20.59 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_3WFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WFO-query.scw PDB file : Tito_Scwrl_3WFO.pdb: