Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMDFNLFMNDIVRQARQEITAAGYTELKTAEEVDEALTKKGTTLVMVNSVCGCAGGIARPAAYHSVHYDKRPDQLVTVFAGQDKEATARARDYFEGYPPSSPSFAIL-KDGKIMKMVERHEIEGHEP------MAVVAKLQEAFEEYCEEV- 1SEN Chain:A ((22-155)) LGKGFG-----------------DHIHWRTLEDGKKEAAASGLPLMVIIHKSWCGACKALKPKFAESTEISELSHNFVMVNLEDEEEPKD--EDFSPDGGYIPRILFLDPSGKVHPEIINENGNPSYKYFYVSAEQVVQGMKEAQERLTGDAF

General information: TITO was launched using: RESULT: Template: 1SEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 606 -29590 -48.83 -234.84 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -48.83 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_1SEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SEN-query.scw PDB file : Tito_Scwrl_1SEN.pdb: