Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERL-----VG-----G-AGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDS---GTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS 1WIN Chain:A ((11-129)) ------------------------------------------------------------------------------------------------------------------------------SLEIMTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVACEQFLGKNVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREV-AAPDVGRMGIEILSFTIKDVYDKVDYL---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1WIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 400 -4148 -10.37 -39.50 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -10.37 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_1WIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WIN-query.scw PDB file : Tito_Scwrl_1WIN.pdb: