Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIGIFGGTFDPPHNGHLLMANEVLYQAGLDEIWFMPNQIPPHKQNEDYTDSFHRVEMLKLAIQSNPSFKLELVEMEREGPSYTFDTVSLLKQRYPNDQLFFIIGADMIEYLPKWYKLDELLNLIQFIGVKRPGFHVETPYPLLFADVPEFEVSSTMIRERFKSKKPTDYLIPDKVKKYVEENGLYES 3MMX Chain:H ((3-189)) MRKIGIIGGTFDPPHYGHLLIANEVYHALNLEEVWFLPNQI--------ITSVESRLQMLELATEAEEHFSICLEELS----SYTYDTMLQLTKKYPDVQFHFIIGGDMVEYLPKWYNIEALLDLVTFVGVARPGYKLRTPYPITTVEIPEFAVSSSLLRERYKEKKTCKYLLPEKVQVYIERNGLY--

General information: TITO was launched using: RESULT: Template: 3MMX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 843 -149570 -177.43 -854.69 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain H : 0.89 3D Compatibility (PKB) : -177.43 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.632

(partial model without unconserved sides chains): PDB file : Tito_3MMX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MMX-query.scw PDB file : Tito_Scwrl_3MMX.pdb: