Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEA---LPAGEQFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVK-AAWPGRLELVQE----HPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLE---TIAHAIHFASFDFPRA--SLAKDLYD---ASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK 1J6U Chain:A ((101-431)) ---------------------------------------KKEEFAVTGTDGKTTTTAMVAHVLKHLRKSPTVF-------------LGGIMD---------------------------SLEHG-------NY-EK-GNGPVVYELDESE--EFFSEFSPNYLIITNARGDHLENYGNSLTRYRSAFEKISRNTDLVVTFAEDELTSH-LGD---------VTFGVKKGTYTLEMRSASRAEQKAMVEKNGKRYLELKLKVPGFHNVLNALAVIALFDSLG------YDLAPVLEALEEFRGVHRRFSIAFHDPETNIYVIDDYAHTPDEIRNLLQTAKEVFENEKIVVIFQPHR--------GNFAKALQLADEVVVTEVYD---SGKMIWDSLKSLGKEAYFVEKLPELEKV----I--SVSENTVFLFVGAGDI---------

General information: TITO was launched using: RESULT: Template: 1J6U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1635 -9536 -5.83 -32.00 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -5.83 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_1J6U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J6U-query.scw PDB file : Tito_Scwrl_1J6U.pdb: