Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKKAVPSMPDVHHVSLDLLKDEVAELVKLGIQSVIVFGIPEEKDDCGT----QAYHDHGIVQKAITEIKEHFPEMVVVADTCLCEYTDHGHCGLV--KDGVILNDESLELLAQTAVSQAKAGADIIAPSNMMDGFVTVIREALDKEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFG-DRKTYQMDPANRMEALREAQSDVEEGADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVKAAAQNGWIKEKEIVLEILTSMKRAGADLIITYHAKDAAKWLAE 3OBK Chain:C ((22-343)) ------RPRRNRKNRAVRQLVQENLVKPSSLIYPLFVHDE-ETSVPIPSMPGQSRLSMEDLLKEVGEARSYGIKAFMLF--PKVDDELKSVMAEESYNPDGLLPRAIMALKEAFPDVLLLADVALDPYSSMGHDGVVDEQSGKIVNDLTVHQLCKQAITLARAGADMVCPSDMMDGRVSAIRESLDMEGCTDTSILAYSCKYASSFYGPFRDALDSHMVGGTDKKTYQMDPSNSREAEREAEADASEGADMLMVKPGLPYLDVLAKIREKSKLPMVAYHVSGEYAMLKAAAEKGYISEKDTVLEVLKSFRRAGADAVATYYAKEAAKWMVE

General information: TITO was launched using: RESULT: Template: 3OBK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1722 -34188 -19.85 -108.53 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain C : 0.84 3D Compatibility (PKB) : -19.85 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.647

(partial model without unconserved sides chains): PDB file : Tito_3OBK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OBK-query.scw PDB file : Tito_Scwrl_3OBK.pdb: