Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKKVIVLGEIRDGELRNVTFEAIAAGRTISGDGEVIGVLIGENVQS-IAQELIHYGADKVLTAEDPKLKTYTADGYSQVMRGVIEQENPDSVIFGHTAMGKDLSPKLAARLQTGLISDAIDVSVTGGNVVFTRPIYSGKAFERVISTDPMIFATIRPNNIQASEKDTSRSGSIESIDVSLTDLRTVIQEVVKKTADGVDLSEAKIIVAGGRGVKSKEGFQPLQELAEVLGAAVGASRGACDADYCDYALQIGQTGKVVTPDLYIACGISGAIQHLAGMSNSKVIVAINKDPEADIFKIADYGIVGDLFEVVPLLTEEFKQLNIHS 1EFV Chain:A ((2-313)) --QSTLVIAEHANDSLAPITLNTITAATRLGG--EVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPD---TFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGS-ASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK------

General information: TITO was launched using: RESULT: Template: 1EFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1811 -20392 -11.26 -65.57 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -11.26 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_1EFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFV-query.scw PDB file : Tito_Scwrl_1EFV.pdb: