Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------MIPIPSAIDGQSFLLQELEQVMKPLGYVINGGWEYDHGYFDYKIDDRDGYLFL-RIPVNAVQGSLDERGAAVRIGTPFMLRQ--VFQADVDDHAE-GGPFQSLFNQFSEPERRDAEIDPAFLDIGASLVKELEDVLLH------------------------------------------------------------------------------ 4KL0 Chain:A ((7-374)) VLTPTEAAVLRELRLHRPQLPLDTLLFTDPNKDPDDVVTYTIAKQLQADGFLRLTDVVVTLGDADMRSQRAQLAKGVFDRLALPDVRVARGQDYPMTSTQAREHSKFLAEGAALRAAPDAVHTDGVRAMCERLATSPHKLGMVVIAGMTDASALLAEAGDLVREKVASITIMGGIDPARLV--QPDTRAYNNATDIHAARALYRRAQQLGIPLRILTKEAAYKAAVPPAFYEGIARNGHPVGEYLRDV-----QKNALKGLWEGIQANLIPGLDTAWFFRTFVALSFDAIWPQVTKLNLYDPLTLLAALPGTARLLFQPTPMHREGASPVEHVGHAEVVRPEKARLLLSALAKAALV

General information: TITO was launched using: RESULT: Template: 4KL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 512 -7693 -15.02 -60.57 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -15.02 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.115

(partial model without unconserved sides chains): PDB file : Tito_4KL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KL0-query.scw PDB file : Tito_Scwrl_4KL0.pdb: