Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFRFTKMHGLGNSYIYVNQFEEQLPEEKLSEIAIQVSSVYTGIGSDGMILICPS-DQAPVKMRIFNNDGSEGKNCGNGLRCVAKYAYEHKLVE-ETSFLIETLSGLVKAEVQVENGKVNVVTVDMGEPRLTKSELPMLDGGEE---HTINETMAFGEVELTGTAVSMGNPHIVFPIADIEQ------APLTTLGPVIEKDPRFPEGINVEFVETVNEQELHFRVWERGSGITQACGTGACAAAVASVLNGVSKRNQDITVHLAGGDLVINWKDN-GHVMMTGPAETVCEGVYFL 3EJX Chain:A ((25-311)) -GVLHFVKYHGLGNDFILVDNRDSSEP-KITQEQAAKLCDRNFGVGADGVIFAMPGVNGTDYAMRIFNSDGSEPEMCGNGVRCFARFIAELENLQGKHSFTIHTGAGLIVPEIQD----DGQVKVDMGTPILKAQDVPT-KLSGNKGEAVVEAELVVDGVSWNVTCVSMGNPHCITFGKKGGPNLKVDDLNLPEIGPKFEHHEMFPARTNTEFVEVLSRSHLKMRVWERGAGATLACGTGACALVVAAVLEGRAD--RKCTVDLPGGPLEIEWKQEDNHIYMTGPAEAVFYGSALL

General information: TITO was launched using: RESULT: Template: 3EJX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1740 -40832 -23.47 -148.48 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -23.47 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_3EJX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EJX-query.scw PDB file : Tito_Scwrl_3EJX.pdb: