Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLNLQGKTALVTGSTSGIGKAIASSLAEEGAAVIINGRREEKVNQTIDELKTQHAEAVLYPAAFDLGTEEGCNELFQA----YPEVDILVNNLGIFEPAEYFDIPDDEWFRFFEVNIMSGVRLTRRYLHNMIEKKEGRVIFIASEAAIMPSQEMAHYSATKTMQLSISRSLAELATGTNVTVNTVMPGSTLTEGVETMLNSLYPGENLTVQEAEARFMKENRPTSIIQRLIRPEEIAHFVTFLSSPLSSAINGAALRADGGLVRSVF 3RRO Chain:B ((8-248)) --MNLEGKVALVTGASRGIGKAIAELLAERGAKVIGTATSESGA-QAISDYLGDNGKGM----ALNVTNPESIEAVLKAITDEFGGVDILVNNAGITRDNLLMRMKEEEWSDIMETNLTSIFRLSKAVLRGMMKKRQGRIINVGSVVGTMGNAGQANYAAAKAGVIGFTKSMAREVASRGVTVNTVAPGF-----IETDMTKALNDEQRTATLAQV-------PAG---RLGDPREIASAVAFLASPEAAYITGETLHVNGGM-----

General information: TITO was launched using: RESULT: Template: 3RRO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1294 -58073 -44.88 -245.03 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -44.88 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3RRO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RRO-query.scw PDB file : Tito_Scwrl_3RRO.pdb: