Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVVTDSTAYIPKEMREQHQIHMIPLQVVFRE-ETYREE-IELDWKSFYEEVKKHNELPTTSQPPIGELVALYEELGK-SYDAVISIHLSSGISGTFSSAAAADSMVDNIDVYPFDSEISCLAQGFYALKAAELIKNGASSPEDIIKELEEMKK--TVRAYFMVDDLAHLQRGGRLSSAQAFIGSLLKVKPILHFDNKVIVPFEKIRTRKKAISRIYELLDEDASKGLPMRAAVIHANREEEAAKIIEELSAKYPHVEFYNSYFGAVIGTHLGEGALGICWCFK 1MGP Chain:A ((26-309)) MKVKILVDSTADVPFSWMEKYDIDSIPLYVVWEDGRSEPDEREPEEIMNFYKRIREAGSVPKTSQPSVEDFKKRYLKYKEEDYDVVLVLTLSSKLSGTYNSAVLASKEV-DIPVYVVDTLLASGAIPLPARVAREMLENGAT-IEEVLKKLDERMKNKDFKAIFYVSNFDYLVKGGRV---------LLKIRVCLHIENGELIPYRKVRGDKKAIEALIEKLREDTPEGSKLRVIGVHADNEAGVVELLNTLRKSYEVVDEIISPMGKVITTHVGPGTVGFGIEVL

General information: TITO was launched using: RESULT: Template: 1MGP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1566 14606 9.33 54.09 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 9.33 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_1MGP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MGP-query.scw PDB file : Tito_Scwrl_1MGP.pdb: