Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTASKFSVMFFMLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTY--------QNLLPQSEDSQAS----GKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVI---LLGEKLMR-------------NQNVDQVLNHFIRDDEL----RRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKIMIPLRIGRAS-----VYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVGFLSADETQTLSWVMGTIQGGVMPTTDKGHPITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTEDQNPNE-NSFSKSYLH-RLENIFEKKLERDVKQVMDKLQ-HEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 4RNZ Chain:A ((1-369)) -MERLVWDKLTLLGFLEKNHIPQKLYYNLSSQDKELSAEIQSNVTYYTLRDANNTLIQALIPISQDLQIHIYKKGEDYFLDFIPIIFTRKEKTLLLSLQTSP-YQDIIKATNDPLLANQLMNAYKKSVPFKRLVKNDKIAIVYTRDYRVGQAFGQPTIKMAMVSSRSNQYYLFSHSNGHYYDSKAQEVAGFLLETPVKYTRISSPFSYGRFHRPHYGVDYAAKHGSLIHSASD--GRVGFMG---VKAGYGKVVEIHL-NELRLVYAHMSAFANGLKKGS--------FVKKGQ-IIGRVGSTG-PHLHFGVYKNSRPI---NPLGYIRTAKSKLHGKQREVFLEKAQRSKQKLEELLKTHSFEKNSFYLLEGFLEHHHH----------------------------------

General information: TITO was launched using: RESULT: Template: 4RNZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1663 69659 41.89 218.37 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 41.89 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_4RNZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNZ-query.scw PDB file : Tito_Scwrl_4RNZ.pdb: