Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MINSRLAVAIHILSLISMDEKTSS---EIIADSVNTNPVVVRRMISLLKKADILTSRAGVPG-ASLKKDPADISLLEVYRAVQKQEELFAV-HENPNPKCPVGKK-IQNALDETFESVQRAMENELASKSLKDVM-NHLF 3LWF Chain:A ((16-159)) YFQGMKITTKGRYGLTITLELAKRIGDGPISLRSIAQDKNLSEHYLEQLIGPLRNAGIVKSIRGAHGGYVLNGDPEKITAGDIIRTLEGPIVLVESMEDEEAAQRELWTRMRNAVRDVLDQTTLSDLLKHSTDSELTDGYMFYI-

General information: TITO was launched using: RESULT: Template: 3LWF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 480 41169 85.77 311.88 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 85.77 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3LWF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LWF-query.scw PDB file : Tito_Scwrl_3LWF.pdb: