Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYA----ISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCG-SPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 4UXJ Chain:D ((12-182)) -------GRIELIIGPMFAGKTTELMRRVKREIHARRSCFVIKY------------------LRAQAAVSQLTEVRDTWKRF----DVLAIDEGQFF-SDLVDFCNTAADAGKVVMVSALDGDYRRKPFGQICELVPYCEAVDKLTAVCMMCHEQPACFTRRTVNVEQQE------LIGGADMYIATCR-----------

General information: TITO was launched using: RESULT: Template: 4UXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 724 -15093 -20.85 -101.98 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -20.85 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_4UXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UXJ-query.scw PDB file : Tito_Scwrl_4UXJ.pdb: