Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLTDKTVLITGGASGIGYAAVQAFLNQQANVVV---ADIDEAQGEAMIRKENNDRLHFVHTDITDEPACQNAIRSAVDKFGGLDVLINNAGIEIVAPIHEMELSNWNKVLNVNLTGMFLMSKHALKYMLKSG-KGNIINTCSVGGVVAWPDIPAYNASKGGVLQLTRSMAVDYAKHNIRVNCVCPGIIDTPLNEKSFLENNEGTLEEIKKEKAKVNPLLRLGKPEEIANVMLFLASDLSSYMTGSAITADGGYTAQ 1GCO Chain:E ((4-251)) -DLEGKVVVITGSSTGLGKSMAIRFATEKAKVVVNYRSKEDEANSVLEEIKKVGGEAIAVKGDVTVESDVINLVQSAIKEFGKLDVMINNAGLENPVSSHEMSLSDWNKVIDTNLTGAFLGSREAIKYFVENDIKGTVINMSSVHEKIPWPLFVHYAASKGGMKLMTETLALEYAPKGIRVNNIGPGAINTPINAEKFAD------PEQRADVESMIPMGYIGEPEEIAAVAAWLASSEASYVTGITLFADGGMT--

General information: TITO was launched using: RESULT: Template: 1GCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1332 -26334 -19.77 -107.92 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain E : 0.80 3D Compatibility (PKB) : -19.77 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_1GCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GCO-query.scw PDB file : Tito_Scwrl_1GCO.pdb: