Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKGLGKGINALFNQVDLSEETVEEIKIADLRPNPYQPRKHFDDEALAELKESVLQHGILQPLIVRKSLKGYDIVAGERRFRAAKLAGLDTVPAIVRELSEALMREIALLENLQREDLSPLEEAQAYDSLLKHLDLTQEQLAKRLGKSRPHIANHLRLLTLPENIQQLIAEGTL-SMGHGRTLLGLK--NKNKLEPLVQKVIAEQLNVRQLEQLIQQLNQNVPRETKKKEPVKDAVLKERESYLQNYFGTTVNIKRQKKKGKIEIEFFSNEDLDRILELLSERES 1R71 Chain:A ((23-134)) -----------------------------------------------------------------------------------------------------E---ADQVIENLQRNELTPREIADFIGRELAK-GKKKGDIAKEIGKSPAFITQHVTLLDLPEKIADAFNTGRVRDVTVVNELVTAFKKRPEEVEAWLDDDT-QEITRGTVKLLREFL-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1R71.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 356 -25203 -70.79 -231.22 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -70.79 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.701

(partial model without unconserved sides chains): PDB file : Tito_1R71.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R71-query.scw PDB file : Tito_Scwrl_1R71.pdb: