Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------------MKLSLFIIAVLMPVILLSACSDHAEEHA-----------------SINTKKTVENITDVRKTAKTSIDWTKPSGGEYPDIK-QKHVWIDVNVKEQKAYIKEGSNTIYTMMISSGLDQTKDDATPKGTFYVEPERGEWFFSE----GYQEGAEYWVSWKNHGEFLFHSVPMTKDQKVIKTEAEKLGTKASHGCIRLTIPDAKWVYENIPEHTKVVIS 1ZAT Chain:A ((1-250)) KEQLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKYNTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTTADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSG---KPTTPTPAGVFYVWNKEEDATLKGTNGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGG------DLWKTRGSHGCINTPPSVMKELFGMVEKGTPVLVF

General information: TITO was launched using: RESULT: Template: 1ZAT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 836 -63439 -75.88 -342.91 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -75.88 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.349

(partial model without unconserved sides chains): PDB file : Tito_1ZAT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZAT-query.scw PDB file : Tito_Scwrl_1ZAT.pdb: