Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKFDISVLSVAPLRQGETMKQGIDSAVSLAKAVDNMGYKRIWFAEHHNHDAYASAATVSIVQHILANTKDIRVGSGGIMLPNHSPLIVAEQFGTLETLYPNRVDLALGRAPGTDQKTADVIRRSNHNGVFFFEREVNDILRFVGDKS-----VQGE---VRAYPGIGTHVPVFVLGSSTDSAEIAAKLGLPYAFGAQFSPHSMEEALSIYRENFQPSSYL-QEPYVIACINVIAAESIDEASFISASHLQVYIDIYTNN---LSKLIPP-TENFLESLS--Q---F-ELEILHS-RLGYTIMGDRETIRRELIDFQQMYHADELIVLSNIYELSKEIQSYEILKQVVDELFKKRMEQL 1Z69 Chain:A ((1-327)) ---MKFGIEFVPSD--------PALKIAYYAKLSEQQGFDHVWITDHYNN-----RDVYSTLTVLALNTNSIKIGPGVTNSYTRNPAITASSIASIAEISGGRAVLGLGPG--D-KATFDAMGIAWKKPLATTKEAIQAIRDFISGKKVSMDGEMIKFAGAKLAFKA-GNIPIYMGAQGPKMLELAGEIADGVLINA-SHPKDFEVAVEQIKKGAEKAGRDPSEVDVTAYACFSIDKDPVKAVNAAKVVVAFIVAGSPDLVLERHGIPVEAKSQIGAAIAKGDFGALMGGLVTPQMIEAFSICGTPDDCMKRIKD-LEAIGVTQIVAGSPIG--PAKEKAIKLIGKEIIAK--------

General information: TITO was launched using: RESULT: Template: 1Z69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1699 -204994 -120.66 -667.73 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -120.66 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_1Z69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Z69-query.scw PDB file : Tito_Scwrl_1Z69.pdb: