Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKYEIIANEMRNRIKNNVYPIDQPIPDEVSLAKEFNSSRMTMKRALDNLVAEGLLFRKRGHGTFIIQSAIQDDHVHVVSNEILGLTNL---LKDKKIKSKVIQFEVQFPTEEVAAHLSIDQKTPVYYVVRLRIVEGEPYVLEKTYMPTHLIPGINDDVL-HDSIYNHITNVLQLKIAGTHRKIRACKSDHIDQQHLGCKQDDPILEVEHVGFLDTGIPFEYSFSRHRHDKFVVTSVNIRR 3F8M Chain:A ((14-241)) -LKHQVVRAELDRMLD-G-MRIGDPFPAEREIAEQFEVARETVRQALRELLIDGRVER-RGRTTVVARPKIRQPL------GMGSYTEAAKAQGL-SAGRILVAWSDLTADEVLAGVLGVDVGAPVLQLERVLTTDGVRVGLETTKLPAQRYPGLRETFDHEASLYAEIRS-RGIAFTRTVDTIDTALPDAREAALLGADARTPMFLLNRVSYDQDDVAIEQRRSLYRGDRMTFTAVMHA-

General information: TITO was launched using: RESULT: Template: 3F8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1004 -79118 -78.80 -353.21 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -78.80 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_3F8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F8M-query.scw PDB file : Tito_Scwrl_3F8M.pdb: