Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASNRYHSINEIMESQLFYQITTPPKKTQKAQYDPEVEKFLEIIEFVKENGREPQKVPTDLTERSLASRLIGIRKDPDRMEYLKEYDEIGLLEVRSKELTIPKISSIDDILKSGLSALLGDNLNNDITRSIFDTSSLQKV-TTMPEYVAKRKKIKDFGKFEELFKKCHKEITEGKRKILTFKNEQDIQSNSFYILKGVLLYVEDVGERKKAKGKTNARLRCIFENGTESDMLLRSLSAELYKHGRRVTDNEDTLLDNVREDDVSTGFIYVLKSLSTDPQISSIKNLYKIGFTTGSVENRIRNAENQSTYLYAPVEIVTTYQVFNMNASKFETAIHHALANNNLDVSILGANGKMLVPKEWFVVTLEDLQAVIDEIVMMVHLYD 3L2O Chain:B ((55-383)) AAS----------------TLTRLPIDVQLYILSFL--SPHDLCQLGSTNHYWNETVRDPILWRYFLLRD------------LPSWSSVDWKSLPDLEILKKPISEVFFDYMAVYRMCCPYLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEELCPTAGLPQ-----RQIDGIGSGVNFQLNNQ-----HKFNILI-----LYSTTRRYSVI----PQIQKVCEV--------------------------------------VDGFIYVANAEAHKR--------HEWQDEFSHIMAMTDPAFGSSGRPLLVLSCISQGDVKRMPCFYLAHELHLNLLNHPWLVQDTEAE------------TLTGFLNGIEWILEEVES--

General information: TITO was launched using: RESULT: Template: 3L2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1182 -127477 -107.85 -466.95 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -107.85 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.309

(partial model without unconserved sides chains): PDB file : Tito_3L2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L2O-query.scw PDB file : Tito_Scwrl_3L2O.pdb: