Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKKKVNLGV-RDVPTPFSWVSFSLQHLFA-MFGSTILVPKLVGMSPAVALVTSGIGTLAYLLITKGQIPAYLGSSFAFISPIILVKATGGPGAAMVGAFLAGLVYGLIALLIRQLGTGWLMKILPPVVVGPVIIVIGLGLASTAVNMAMYADPNASELVYSLKHFSVAGVTLAITIICAIFLRGFLSLIPVLIGIIGGYLFALTQGIVNFQPVLDAKWFAVPEFIIPFKDYSPSVTLGIAAAMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIMGDSVATILASLIGGPPTTTYGENIGVLAITRVFSVFVIGGAAVIALCFGFIGKISALISSVPSAVMG-GVSFLLFGIIASSGLRMLIDNKIDYENNRNLIITSVILVIGVGGAFIQVSQGGFQVSGMALAAIVGVILNLILPQAKEEQADTSEQHHI 4MUO Chain:A ((1-320)) MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGGVLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANL--------TPLLAILLHKLG-------FPVVVHGVS------------------EDPTR---VLTETIFELMGIT------------------PTL-----------------HGGQAQAKLDEHQPVFMPVGAFCPPLEKQLA----------MRWRMGVR------------------------NSAHTLAKLA-----TPFAEG----EALRLSSVSHPEYIGRVAKFFSDIGGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQAKDPETTAQWIERCL--------------AGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF

General information: TITO was launched using: RESULT: Template: 4MUO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1685 -224686 -133.34 -731.88 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -133.34 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_4MUO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MUO-query.scw PDB file : Tito_Scwrl_4MUO.pdb: