Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIEMK-PERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDTSAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYS-NDKGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKV--PAKIGQYNVQTEVFKAMANAGISVDFFNITPSE--IVYTVAGNKTETA----QRILMDMGYDPMVTRNCAK----------VSAVGAGIMGVPGVTSKIVSALSEK--EIPILQSADSHTTIWVLVHEADMVPAVNALHEVFELSK 3C1M Chain:C ((108-467)) ------------------------------------------------------LIGVAYLGE--LTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDM------EMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGD-GKKSFLNNNLIRDVSVDKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKF----

General information: TITO was launched using: RESULT: Template: 3C1M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1707 -194540 -113.97 -577.27 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -113.97 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_3C1M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C1M-query.scw PDB file : Tito_Scwrl_3C1M.pdb: