Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQNKRKHLQTLFETL-GEKHQFNGTVLAAEGGDILYHHSFGYAEMTEKRPLKTNSLFELASLSKPFTALGIILLEEKGILGYEDKVDRWLPGFPYQGVTIRHLLNHTSGLPDYMGWFFANW--DSHKIAVNQDIVDMLMNEG-LSGYFEPNEGWMYSNTGYVLLAVIIEKASGMSYADFIKTSIFLPAGMNETRVYNRRLSPERIDHYAYGYVYDVHSETYVLPDELEETNYVVYLDGIQGDGTVNSVTSDLFRFDQALYQDDFISKASKESAFSPVRLNNGETIDYGFGWVLQNSPEKGRIVSHSGGWPGYSTMMIRYIDHRKTLIYLSNKEEDTEYEQAILKAAEHILFGQPYDVP------E---------------R---------------------P----A-------DK-K----------------KKAIDTAIYSRYVGSYLLQD-GTAAQVTTENERLYLEIAGQ----LRLELFPSSETRFFLRAL---------SVEVEFTLGEDAAK-SFILYEDGSEEEAVRTK 1EI5 Chain:A ((3-506)) --KFDTSALEAFVRHIPQNYKGPGGVVAVVKDGEVVLQHAWGFADLRTRTPMTLDTRMPICSVSKQFTCAVLLDAVGEPEL-LDDALEAYLDKFEDERPAVRDLCNNQSGLRDYWALS-VLCGADPEGVFLPAQAQSLL--RRLKTTHFEPGSHYSYCNGNFRILADLIEAHTGRTLVDILSERIFAPAGMKRAELISDTA----LFDECTGYEGDTVRGFL---------PAT-NRIQWMGDAGICASLNDMIAWEQFIDATRDDESGLYRRLSGPQTFKDGVAAPYGFGLNLHETGGK-RLTGHGGALRGWRCQRWHCADERLSTIAMFNFEGGAS--EV-AFKLMNIALGVSSSEVSRVEADSAWFGSWLDDETGLVLSLEDAGHGRMKARFGTSPEMMDVVSANEARSAVTTIRRDGETIELVRASENLRLSMKRVKGEAKHDIIGRYHSDELDADLLLVSEGGAIYGAFEGFLGKSDMYPLYSVGSDVWLLPVQRSMDAPSPGEWKLVFRRDDKGEITGLSVG------------

General information: TITO was launched using: RESULT: Template: 1EI5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2324 -3023 -1.30 -7.28 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -1.30 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.356

(partial model without unconserved sides chains): PDB file : Tito_1EI5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EI5-query.scw PDB file : Tito_Scwrl_1EI5.pdb: