Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLTLKKAHAAKIKKGYPLIEKEALAGSAGHMKEGDLVDIVSESGGEFLARGYYGLQNKGVGWTLTRNKHEQIDQAFFLSKLTKAAQARAKLFEAQDTTAFRLFNGEGDGVGGVTIDYYDGYLLIQWYSKGIYTFKDMLISALDEMDLDYKAIYEKKRFDT-A-GQYVEDDDFVKGRRGEFPIIIQENGIQYAVDLNEGAMTGIFLDQRHVRKAIRDRYA-KGKTVLNTFSYTGAFSVAAALGGAEKTTSVDVANRSLAKTIEQFSVNKLDYEAHDIKVMDVFNYFSYAAKKDLRFDLIILDPPSFARTKKRTFSAAKDYKNLLKETIAITADKGVIVASTNSSAFGMKKFKGFIDAAFKETNERYTIIEEFTLPEDFKTISAFPEGNYLKVVLLQKK 4DMG Chain:A ((6-382)) -LRLVVKAGKERKLRNFYPNLYRDEIAAPP---EGVGVAEAVD-AEGHFLAVGYYDPRSRVPFRAFRFDP-GPLNRAFFQGRFARALRRRQGL-----GESHRLVHGEADGLPGLVVDRFGEVLVLQVRSRGMEALREVWLPALLEVVA-PKGVYERSDVEARRQEGLPERVGVVYGEV-PEVLEVEEDGLRFPIPLALAQKTGYYLDQRENRRLFEA-MVRPGERVLDVYSYVGGFALRAARKGA-YALAVDKDLEALGVLDQAALRLGL---RVDIRHGEALPTLRG---LEGPFHHVLLDPPTLVKRPEELPAMKRHLVDLVREALRLLAEEGFLWLSSCSYHLRLEDLLEVARRAAADLGRRLRVHRVTYQPEDHPWSLHIPESLYLKTLVLQD-

General information: TITO was launched using: RESULT: Template: 4DMG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2135 -50208 -23.52 -134.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -23.52 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_4DMG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DMG-query.scw PDB file : Tito_Scwrl_4DMG.pdb: