Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILIAILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSKLLAIDVGVVIFSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIALIGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLGRVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSVLLNILNTISTICLLMAMVALGLNVAFKDLKNRALKPLMTI------IITSICLSSLAFIVVHWLYS 2AFJ Chain:A ((6-231)) ------------------------------------------------GSSARQSTPTSQALYSDFSPPEGLEELLSAPPPDLVAQRHHGWNPKDCSENIDVKEGGLCFERRPVAQSTDGVRGKRGYSRGLHAWEIS------WPLEQRGTHAVVGVAT---------------------------ALAPLQADHY----AALLGSNSESWGWDIGRGK---------------LYHQSKGLEAPQYPAGPQGEQLVVPERLLVVLDMEEGTLGYSIG-----------GTYLGPAFRGLKGRTLYPSVSAVWGQCQVRIRYMGERRVEETRRIHRD

General information: TITO was launched using: RESULT: Template: 2AFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -141763 for 1364 contacts (-103.9/contact) + 2D Compatibility (PS) -22901 + (NN) 5143 + (LL) 13240 1D Compatibility (HY) 6000 + (ID) 1100 Total energy: -141381.0 ( -103.65 by residue) QMean score : 0.024

(partial model without unconserved sides chains): PDB file : Tito_2AFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AFJ-query.scw PDB file : Tito_Scwrl_2AFJ.pdb: