Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKT---IFAFLTGLMMFGTITAASASPDSKNQTAKKPKVQTGIDT---LLPDYKKQLKGKRI-----GLITNPAGVNTSLK---------SSVDILYENPDIKLTALFGPEHGVRGDAQAGDEVGSYID----------EKTGVPVYSLYG----KTKKPTPEMLKNVDILMFDIQDVGTRFYTYIYTMAYAMEAAKENG--IPFMVLDRPNPQGGNHIEGPILE-PEYASFVGLY---PIPLKHGMTIGELAS-----LFNKEFSIDADLTVVKMKHW--------KRKMDFDDTRLPF----VLPSPNMPTVESTFVYPATGLIEGTNISEGRGTTKPFELIGAPFIKSTEL------EETLNSLHLPGVTFRAASFTPTFSKHQGTLCHGVQLY-----VTDRDKFEAVKTGLSVIKTIHDLYPE----------DFEFLSTGSFDKLAGNGWIRTKIENG--TSVENIINSYEKTLQQFSKTRKKYLIY 4IWS Chain:A ((23-515)) MNRSALDFRHFVDHLRRQGDLVDVHTEVDANLEIGAITRRVYERRAPAPLFHNIRDSLPGARVLGAPAGLRADRARAHSRLALHFGLPEHSGPRDIVAMLRAAMRAEPIAPRRLERGPVQENVWLGEQVDLTRFPVPLLHEQDGGRYFGTYGFHVVQTPDGSWDSWSVGRLMLVDRNTLAGPTIPTQ-HIGIIREQWRRLGKPTPWAMALGAPPAALAAAGMPLPEGVSEAGYVGALVGEPVEVVRTQTNGLWVPANTEIVLEGEISLDETALEGPMGEYHGYSFPIGKPQPLFHVHALSFRDQPILPICVAGTPPEENHTIWGTMISAQLLDVAQNAGLPVDMVWCSYEAATCWAVLSIDVQRLAALGTDAAAFAARVAETVFGSHAGHLVPKLILVGNDIDVTEIDQVVWALATRAHPLHDHFAFPQIRDFPMVPYLDAEDKARGSGGRLVINCLYPEQFAGQMRAATASFRHAYPTALRRRVEERWSDYGF

General information: TITO was launched using: RESULT: Template: 4IWS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -32739 for 3045 contacts (-10.8/contact) + 2D Compatibility (PS) -42168 + (NN) -755 + (LL) 92 1D Compatibility (HY) -7200 + (ID) 3800 Total energy: -86570.0 ( -28.43 by residue) QMean score : 0.205

(partial model without unconserved sides chains): PDB file : Tito_4IWS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IWS-query.scw PDB file : Tito_Scwrl_4IWS.pdb: