Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------MFN------TAVKILYRSLIELT----NHRLSSYLIKGFCESKISKP---VIPLFSKHFRLNWDDVDGT------AADYGSLSELFIRQINLERRPV-SKEAHA----------VVSPVDGVVQTVGIINPN--------QTFTVKGKDYSFAELT--GCKSADHQYNG-GYFVVLYLSPRHYHRFHSPISCRYQKLAELGNRSYPVN----QLGLKYG---------------KDVLSKNYRFVYELNSGSRNVLMIPVGAMNINSIVQ---TN-TRTELEIGEELGYFSFG--STVILVFEKDAFQPSAHLAEGQEVQVGELIG----YEE--------------- 3WJ9 Chain:A ((3-412)) QKSQFAYRSSKSIGLVNASENYASPPKFEAISEPARNACYSPNGKLFAYATATQVVINDTESGAKLTQLPAANTYELGFSPLGKYLSTWERPGKEADGTPKQNMKVWNTETGQLVFSFVQRNQTGWNLQYTCDESLAARLVTNEVHFYETGNMSKGPIAKLRVEGISDFALSP--GQNHAVAVFIPEKKGAPASVRTYSIPNFNSPLSQKTFFKADKVQFKWNALGTSLLVLTQDKSNKNYYGETTGQFDLDREGPIHDVCWNADSKEFGIVYGYMPAKTAIFDNRANVVSIIPPAPRNTLIFSPNSRYILLAGFGNLQGSIDIFDAANNMKKITTVEAANCTYCEFSPDSQFLLTAVT-----SPRLRVDNSIKIWHITGAPMFYEEFNELYQAFWRPRPLN

General information: TITO was launched using: RESULT: Template: 3WJ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1067 -80826 -75.75 -315.73 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -75.75 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_3WJ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WJ9-query.scw PDB file : Tito_Scwrl_3WJ9.pdb: