Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKVKRNAPCPCGSGKKYKKCCGSKVVDFPAELAAKEAKQIQEDLVEYAFTVHRESISGFINQHDFLSAMDRQTKDISVFNLGIWGIFFHPLAGEKTIFEEYLQKKGDSITRPKTREIVESWQSMTPALLLLKDLKEGIIHFEDVITAKQFEVEMDASNQDLPPVGSLILGYPIHEAEKAEFFMQFTIFPVKRTEALISKVKKYADAAVKDGKTPEDFMKQEFNNVLFALLAEKDEEPQAEKAEVSTVEWANDLEKETAAAIEEGMSGEEYPTELIPAVIDIWKTFCEKKSPVIRKPEAFAAAVEYYVNAISLNG--ASVSQAKLAKKYGVSASTISSRYKEIESTLQDEADRFAQALSS 3G33 Chain:B ((181-254)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HAQTFLALCATDYTFAMYPPSMIATGSIGAAVQGLG--MSGDELTELLAGITGTEVDCLRACQEQIEAALRES----------

General information: TITO was launched using: RESULT: Template: 3G33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 249 -27917 -112.12 -404.59 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -112.12 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_3G33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G33-query.scw PDB file : Tito_Scwrl_3G33.pdb: