Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPAKTFVILCILFLLDLCFSYIRHEWHS-QNALQDM---PVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDELYKQGRTKKGN---IVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGF--EWGGDWKRF----KDYPHLEMIPN 4MUR Chain:A ((22-182)) ----------MNTLQLINKNHPLKK---NQEPPHLVLAPFSDHDVYLQPEVAKQWERLVRATG-LEKDIRLVSGYRTEKEQRRLWEYSLKENGLAYTKQFVALPGCSEHQIGLAIDVGLKKQEDD-DLICP-HFRDSAAADL--FMQQMMNYGFILRYPEDKQEITGISYEPWHFRYVG-

General information: TITO was launched using: RESULT: Template: 4MUR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 656 -41836 -63.77 -282.67 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -63.77 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_4MUR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MUR-query.scw PDB file : Tito_Scwrl_4MUR.pdb: